HPV genotyping and p16 expression in Xingu Indigenous Park, Brazil

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dc.contributor.authorFreitas, Valéria Grisolia
dc.contributor.authorFocchi, Gustavo R.
dc.contributor.authorPereira, Érica Ribeiro
dc.contributor.authorLevi, J.E.
dc.contributor.authorSpeck, Neila M. de Góis
dc.contributor.authorRibalta, Julisa Chamorro
dc.creator.affilliationUniversidade Federal de São Paulo. Departamento de Ginecologia. Núcleo de Prevenção de Doenças Ginecológicas. São Paulo, SP, Brasilen_US
dc.creator.affilliationUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Patologia. São Paulo, SP, Brasilen_US
dc.creator.affilliationUniversidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Medicina Preventiva. São Paulo, SP, Brasilen_US
dc.creator.affilliationUniversidade de São Paulo. Instituto de Medicina Tropical. São Paulo, SP, Brasilen_US
dc.creator.affilliationUniversidade Federal de São Paulo. Departamento de Ginecologia. Núcleo de Prevenção de Doenças Ginecológicas. São Paulo, SP, Brasilen_US
dc.creator.affilliationUniversidade Federal de São Paulo. Departamento de Ginecologia. Núcleo de Prevenção de Doenças Ginecológicas. São Paulo, SP, Brasilen_US
dc.date.accessioned2022-05-15T12:02:02Z
dc.date.available2022-05-15T12:02:02Z
dc.date.issued2016
dc.description.abstractenThe association between high-risk human papillomavirus (HPV) genotypes and p16 expression in indigenous women from the Xingu Indigenous Park, Brazil, was unknown. This study evaluated p16 expression in women with a histological diagnosis of cervical intraepithelial neoplasia (CIN) 3 or higher and correlated this expression with HPV genotypes to determine possible discrepancies in the expression of this marker. We evaluated 37 previously collected samples with different HPV genotypes and high-grade lesions diagnosed based on cytology, histology, and colposcopy. Immunohistochemical analysis was performed using paraffin-embedded tissue sections and the CINtec® Histology Kit. p16 protein expression was investigated by immunostaining with an anti-p16 antibody. HPV genotyping was performed by reverse hybridization. The age of the study population ranged from 22-75 years (43.81 ± 15.89 years) and parity ranged from 1-11 (5.92 ± 2.58). Thirteen different HPV genotypes were found using the INNO-LiPA kit. Single and multiple infections by HPV were found with prevalence of single infections (P = 0.029). Comparison between HPV genotype and simple or multiple infections was highly significant; it was observed more HPV 52 followed by HPV 16 in single infections (P < 0.001). p16 expression was predominantly diffuse, which was observed in 91.7% of lesions, whereas 8.3% were focal (P < 0.001). HPV 52, HPV 16 and 31 were the most prevalent HPV types in high-grade CIN in these indigenous women. Diffuse p16 expression in high-grade CIN was not influenced by the viral genotype; however, more studies are necessary to further our understanding of this restricted group.en_US
dc.identifier.citationFREITAS, V.G.; FOCCHI, G.R.; PEREIRA, E.R.; LEVI, J.E.; SPECK, N.M.G.; RIBALTA, J.C. HPV genotyping and p16 expression in Xingu Indigenous Park, Brazil. Genetics and Molecular Research, Ribeirão Preto, v. 15, n. 3, p. gmr.15036840, 2016.en_US
dc.identifier.doi10.4238/gmr.15036840
dc.identifier.urihttps://repositorio.bvspovosindigenas.fiocruz.br/handle/bvs/6924
dc.language.isoengen_US
dc.publisherFundação de Pesquisas Cientificas de Ribeirão Pretoen_US
dc.rightsopen accessen_US
dc.subject.decsBrasilen_US
dc.subject.decsSaúde de Populações Indígenasen_US
dc.subject.decsÍndios Sul-Americanosen_US
dc.subject.decsSaúde da Mulheren_US
dc.subject.decsGenótipoen_US
dc.subject.decsAlphapapillomavirusen_US
dc.subject.decsGenes p16en_US
dc.subject.enBrazilen_US
dc.subject.enHealth of Indigenous Peoplesen_US
dc.subject.enIndians, South Americanen_US
dc.subject.enGenotypeen_US
dc.subject.enGenes, p16en_US
dc.subject.enAlphapapillomavirusen_US
dc.subject.enWomen's Healthen_US
dc.subject.otherMato Grossoen_US
dc.subject.otherRegião Amazônicaen_US
dc.subject.otherRegião Centro-Oesteen_US
dc.subject.otherXingúen_US
dc.titleHPV genotyping and p16 expression in Xingu Indigenous Park, Brazilen_US
dc.typeArticleen_US
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